Paratope diversity in the human antibody response to Bacillus anthracis protective antigen
نویسندگان
چکیده
منابع مشابه
Genetic diversity in the protective antigen gene of Bacillus anthracis.
Bacillus anthracis is a gram-positive spore-forming bacterium that causes the disease anthrax. The anthrax toxin contains three components, including the protective antigen (PA), which binds to eucaryotic cell surface receptors and mediates the transport of toxins into the cell. In this study, the entire 2,294-nucleotide protective antigen gene (pag) was sequenced from 26 of the most diverse B....
متن کاملPreparation and Evaluation of Human-Murine Chimeric Antibody against Protective Antigen of Bacillus anthracis
The aim of this research is to develop a human/murine chimeric Fab antibody which neutralizes the anthrax toxin, protective antigen (PA). The chimeric Fab was constructed using variable regions of murine anti-PA monoclonal antibody in combination with constant regions of human IgG. The chimeric PA6-Fab was expressed in E. coli. BL21 and evaluated by ELISA and co-immunoprecipitation- mass spectr...
متن کاملIn-silico Design and Antibody Response to the Peptide Sequences from Protective Antigen and Lethal Factor Toxins of Bacillus anthracis
Bacillus anthracis is the only pathogen known to be highly infectious with potential biothreat in the family Bacillaceae. The organism in its endospore form has the potential to get aerosolized and infect large population resulting in panic and mass causality (Wilkening, 2006). Because of this reason, Center for Disease control and prevention (CDC) has listed Bacillus anthracis as category A ag...
متن کاملA monoclonal antibody to Bacillus anthracis protective antigen defines a neutralizing epitope in domain 1.
Antibody (Ab) responses to Bacillus anthracis toxins are protective, but relatively few protective monoclonal antibodies (MAbs) have been reported. Protective antigen (PA) is essential for the action of B. anthracis lethal toxin (LeTx) and edema toxin. In this study, we generated two MAbs to PA, MAbs 7.5G and 10F4. These MAbs did not compete for binding to PA, consistent with specificities for ...
متن کاملCrystallization of the protective antigen protein of Bacillus anthracis.
The protective antigen protein, one of the three separate proteins constituting the exotoxin system of Bacillus anthracis, has been crystallized in a form suitable for structural studies. The crystal form which is most amenable to x-ray analysis is orthorhombic, space group P2(1)2(1)2(1), a = 101.1 A, b = 95.4 A, c = 87.3 A, with one protective antigen monomer/asymmetric unit. The crystals diff...
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ژورنال
عنوان ژورنال: Molecular Immunology
سال: 2008
ISSN: 0161-5890
DOI: 10.1016/j.molimm.2007.06.159